Hotspot

psbZ-trnG-GCC

LSC · 35865-36311 · 447 bp

Back to Parietaria

Pi 0.0394
MarkerSeek score 66.30
Primer available yes
Label psbZ-trnG-GCC

Feature Metadata

label_name
psbZ-trnG-GCC
feature_type
igs
region
LSC
length_bp
447
variable_sites
31
pi
0.0394
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.99
markerseek_score
66.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbZ-trnG-GCC_p1 CCGGTACATCATTATGGATTGGA TCTTCTCCTTGGCAAAGAGA 43.5 45.0 58.1 56.1 552-559 bp 1.0 46.6
psbZ-trnG-GCC_p2 TCCGGTACATCATTATGGATTGG TCTTCTCCTTGGCAAAGAGA 43.5 45.0 58.1 56.1 553-560 bp 1.0 46.6
psbZ-trnG-GCC_p3 CCGGTACATCATTATGGATTGG TCTTCTCCTTGGCAAAGAGA 45.5 45.0 56.7 56.1 552-559 bp 1.0 46.6
psbZ-trnG-GCC_p4 CCGGTACATCATTATGGATTGGA TCTTCTCCTTGGCAAAGAGAA 43.5 42.9 58.1 56.9 552-559 bp 1.0 46.6
psbZ-trnG-GCC_p5 TCCGGTACATCATTATGGATTGG TCTTCTCCTTGGCAAAGAGAA 43.5 42.9 58.1 56.9 553-560 bp 1.0 46.6