Hotspot

rpoB-trnC-GCA

LSC · 26413-27515 · 1103 bp

Back to Parietaria

Pi 0.0302

MarkerSeek score 68.00

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 1103
variable_sites: 59
pi: 0.0302
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 68.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	CCAAATTAACCCCGCGGATA	TAGTGTAGTGGGGGCTATCG	50.0	55.0	58.0	58.0	1651-1660 bp	0.4	58.5
rpoB-trnC-GCA_p2	GATACCGGGGCTTTGCAATA	TAGTGTAGTGGGGGCTATCG	50.0	55.0	58.0	58.0	1783-1792 bp	0.4	58.3
rpoB-trnC-GCA_p3	CGAGCCCATATCCTTGCTTT	TAGTGTAGTGGGGGCTATCG	50.0	55.0	58.0	58.0	1899-1908 bp	0.4	58.2
rpoB-trnC-GCA_p4	CCGGGGCTTTGCAATATTTG	TAGTGTAGTGGGGGCTATCG	50.0	55.0	58.1	58.0	1779-1788 bp	0.4	58.0
rpoB-trnC-GCA_p5	TACCGGATCTCCACCTACAC	TAGTGTAGTGGGGGCTATCG	55.0	55.0	57.9	58.0	2101-2110 bp	0.4	57.8