Hotspot

rps16-trnQ-UUG

LSC · 6191-6875 · 685 bp

Back to Parietaria

Pi 0.0409
MarkerSeek score 67.10
Primer available yes
Label rps16-trnQ-UUG

Feature Metadata

label_name
rps16-trnQ-UUG
feature_type
igs
region
LSC
length_bp
685
variable_sites
50
pi
0.0409
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
67.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-UUG_p1 AAGTCGCACGTTGCTTTCTA GAGGTTCGAATCCTTCCGTC 45.0 55.0 58.1 58.1 758-794 bp 1.0 83.8
rps16-trnQ-UUG_p2 AAGTCGCACGTTGCTTTCTA TTCGGAGGTTCGAATCCTTC 45.0 50.0 58.1 57.3 762-798 bp 1.0 79.1
rps16-trnQ-UUG_p3 CGTTGCTTTCTACCACATCG GAGGTTCGAATCCTTCCGTC 50.0 55.0 57.2 58.1 750-786 bp 1.0 78.7
rps16-trnQ-UUG_p4 AGTCGCACGTTGCTTTCTAC GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.9 58.1 757-793 bp 1.0 78.0
rps16-trnQ-UUG_p5 GCACGTTGCTTTCTACCACA GAGGTTCGAATCCTTCCGTC 50.0 55.0 59.1 58.1 753-789 bp 1.0 76.5