Hotspot

psbA

LSC · 92-1153 · 1062 bp

Back to Pariana

Pi 0.00471

MarkerSeek score 46.60

Primer available yes

Label psbA

Feature Metadata

label_name: psbA
feature_type: gene
region: LSC
length_bp: 1062
variable_sites: 5
pi: 0.00471
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 46.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbA_p1	TCTGGCTTTCCTTCCTTCAA	ATCACACAGCGAGCGTATTT	45.0	45.0	56.7	58.0	1714 bp	1.0	69.6
psbA_p2	TTCTGGCTTTCCTTCCTTCA	ATCACACAGCGAGCGTATTT	45.0	45.0	56.7	58.0	1715 bp	1.0	69.6
psbA_p3	TCTGGCTTTCCTTCCTTCAA	CATAGGGAAAGTCGTGTGCA	45.0	50.0	56.7	57.9	1474 bp	1.0	68.9
psbA_p4	TTCTGGCTTTCCTTCCTTCA	CATAGGGAAAGTCGTGTGCA	45.0	50.0	56.7	57.9	1475 bp	1.0	68.9
psbA_p5	TCTGGCTTTCCTTCCTTCAA	AATACGTGTGCTTGGGAGTC	45.0	50.0	56.7	57.9	1159 bp	1.0	68.9