Hotspot

ndhF

SSC · 104932-107151 · 2220 bp

Back to Pariana

Pi 0.00631

MarkerSeek score 52.80

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2220
variable_sites: 14
pi: 0.00631
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 52.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	TAGGAAGGGGAATAAGGGGG	GGTTTGGTGAATCGGAAACAG	55.0	47.6	57.5	57.7	2342 bp	1.0	66.0
ndhF_p2	TAGGAAGGGGAATAAGGGGG	TGGTTTGGTGAATCGGAAACA	55.0	42.9	57.5	58.3	2343 bp	1.0	65.5
ndhF_p3	TAGGAAGGGGAATAAGGGGG	GGTTTGGTGAATCGGAAACA	55.0	45.0	57.5	56.5	2342 bp	1.0	64.6
ndhF_p4	TAGGAAGGGGAATAAGGGGG	TGGTTTGGTGAATCGGAAAC	55.0	45.0	57.5	56.5	2343 bp	1.0	64.6
ndhF_p5	GGAGCTCGGGGAATTCCTTA	GGTTTGGTGAATCGGAAACAG	55.0	47.6	58.9	57.7	2310 bp	1.0	63.3