Hotspot

trnD-GUC-psbM

LSC · 16662-17681 · 1020 bp

Back to Pariana

Pi 0.00211
MarkerSeek score 64.20
Primer available yes
Label trnD-GUC-psbM

Feature Metadata

label_name
trnD-GUC-psbM
feature_type
igs
region
LSC
length_bp
1020
variable_sites
2
pi
0.00211
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.93
markerseek_score
64.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnD-GUC-psbM_p1 TTCAATTGGTCAGAGCACCG TCGTTTTGGCTGACTGTTTT 50.0 40.0 58.5 56.4 1113-1185 bp 1.0 67.5
trnD-GUC-psbM_p2 TTCAATTGGTCAGAGCACCG AATCGTTTTGGCTGACTGTT 50.0 40.0 58.5 56.2 1115-1187 bp 1.0 65.7
trnD-GUC-psbM_p3 TTCAATTGGTCAGAGCACCG ATCGTTTTGGCTGACTGTTT 50.0 40.0 58.5 56.2 1114-1186 bp 1.0 65.7
trnD-GUC-psbM_p4 TTCAATTGGTCAGAGCACCG TCGTTTTGGCTGACTGTTTTT 50.0 38.1 58.5 57.1 1113-1185 bp 1.0 65.4
trnD-GUC-psbM_p5 TTCAATTGGTCAGAGCACCG ATCGTTTTGGCTGACTGTTTT 50.0 38.1 58.5 56.9 1114-1186 bp 1.0 63.7