Hotspot

rps2

LSC · 16201-16911 · 711 bp

Back to Parathesis

Pi 0.00141

MarkerSeek score 59.60

Primer available yes

Label rps2

Feature Metadata

label_name: rps2
feature_type: gene
region: LSC
length_bp: 711
variable_sites: 1
pi: 0.00141
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps2_p1	GAGGTTCCCAAGTTGTAACCA	TTTTTGAAAAGGGGAGGGGG	47.6	50.0	57.8	57.9	811 bp	1.0	69.5
rps2_p2	TGAGGTTCCCAAGTTGTAACC	TTTTTGAAAAGGGGAGGGGG	47.6	50.0	57.8	57.9	812 bp	1.0	69.5
rps2_p3	AGGTTCCCAAGTTGTAACCA	TTTTTGAAAAGGGGAGGGGG	45.0	50.0	56.6	57.9	810 bp	1.0	67.7
rps2_p4	GAGGTTCCCAAGTTGTAACCA	ATTTTTCAGGACACCTCGCC	47.6	50.0	57.8	58.5	836 bp	1.0	66.6
rps2_p5	TGAGGTTCCCAAGTTGTAACC	ATTTTTCAGGACACCTCGCC	47.6	50.0	57.8	58.5	837 bp	1.0	66.6