Hotspot

rpl16

LSC · 83441-84832 · 1392 bp

Back to Parathesis

Pi 0.00144

MarkerSeek score 59.60

Primer available yes

Label rpl16

Feature Metadata

label_name: rpl16
feature_type: gene
region: LSC
length_bp: 1392
variable_sites: 2
pi: 0.00144
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16_p1	TGCCTTTTTCATTCCCAAGC	AGAGTTCTTAATTCCGTAATTCT	45.0	30.4	57.2	53.2	1511-1512 bp	1.0	40.1
rpl16_p2	GCCTTTTTCATTCCCAAGCTC	AGAGTTCTTAATTCCGTAATTCT	47.6	30.4	58.0	53.2	1510-1511 bp	1.0	40.1
rpl16_p3	TGCCTTTTTCATTCCCAAGC	AAGAGTTCTTAATTCCGTAATTCT	45.0	29.2	57.2	54.0	1512-1513 bp	1.0	40.1
rpl16_p4	TGCCTTTTTCATTCCCAAGC	AGAGTTCTTAATTCCGTAATTCTT	45.0	29.2	57.2	54.0	1511-1512 bp	1.0	40.1
rpl16_p5	TTGCCTTTTTCATTCCCAAGC	AGAGTTCTTAATTCCGTAATTCT	42.9	30.4	57.9	53.2	1512-1513 bp	1.0	40.1