Hotspot

matK

LSC · 2079-3605 · 1527 bp

Back to Parathesis

Pi 0.00131

MarkerSeek score 59.40

Primer available yes

Label matK

Feature Metadata

label_name: matK
feature_type: gene
region: LSC
length_bp: 1527
variable_sites: 2
pi: 0.00131
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
matK_p1	TTCTCATTTACACGGTCTGA	GGCCTCTTTTCTTACTCGCA	40.0	50.0	54.0	57.9	1659 bp	1.0	47.7
matK_p2	AGCCTTAACTAGATGCTACATGA	GGCCTCTTTTCTTACTCGCA	39.1	50.0	56.9	57.9	1747 bp	1.0	46.3
matK_p3	TCTCATTTACACGGTCTGAT	GGCCTCTTTTCTTACTCGCA	40.0	50.0	53.8	57.9	1658 bp	1.0	45.8
matK_p4	TTCTCATTTACACGGTCTGA	AGGCCTCTTTTCTTACTCGC	40.0	50.0	54.0	57.6	1660 bp	1.0	45.4
matK_p5	TCTCATTTACACGGTCTGATAACA	GGCCTCTTTTCTTACTCGCA	37.5	50.0	57.7	57.9	1658 bp	1.0	45.1