Hotspot

rpl32-trnL-UAG

SSC · 115589-116398 · 810 bp

Back to Parathesis

Pi 0.00741

MarkerSeek score 63.00

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 810
variable_sites: 6
pi: 0.00741
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	TAAATCTCTTTCCACCGGGC	TCCTAAGAGCAGCGTGTCTA	50.0	50.0	57.6	57.9	913 bp	1.0	76.2
rpl32-trnL-UAG_p2	TAAATCTCTTTCCACCGGGC	AGCAGCGTGTCTACCAATTT	50.0	45.0	57.6	57.8	906 bp	1.0	75.7
rpl32-trnL-UAG_p3	TAAATCTCTTTCCACCGGGC	CTAAGAGCAGCGTGTCTACC	50.0	55.0	57.6	57.8	911 bp	1.0	75.7
rpl32-trnL-UAG_p4	TAAATCTCTTTCCACCGGGC	CCTAAGAGCAGCGTGTCTAC	50.0	55.0	57.6	57.8	912 bp	1.0	75.6
rpl32-trnL-UAG_p5	ACCGGGCAGTCAAAAAGTTT	TCCTAAGAGCAGCGTGTCTA	45.0	50.0	58.5	57.9	900 bp	1.0	75.4