Hotspot

ndhF-rpl32

SSC · 114543-115420 · 878 bp

Back to Parathesis

Pi 0.00114
MarkerSeek score 59.40
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
878
variable_sites
1
pi
0.00114
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
59.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 GACGAAGCCTTTTTGTTGGG AAAGCCGCCCAATATCCTTG 50.0 50.0 57.9 58.6 1076-1086 bp 1.0 74.2
ndhF-rpl32_p2 GACGAAGCCTTTTTGTTGGG CCGCCCAATATCCTTGCTTT 50.0 50.0 57.9 58.6 1072-1082 bp 1.0 74.2
ndhF-rpl32_p3 CGACGAAGCCTTTTTGTTGG AAAGCCGCCCAATATCCTTG 50.0 50.0 58.2 58.6 1077-1087 bp 1.0 73.3
ndhF-rpl32_p4 CGACGAAGCCTTTTTGTTGG CCGCCCAATATCCTTGCTTT 50.0 50.0 58.2 58.6 1073-1083 bp 1.0 73.3
ndhF-rpl32_p5 CCACATACGACGAAGCCTTT CCGCCCAATATCCTTGCTTT 50.0 50.0 58.3 58.6 1080-1090 bp 1.0 73.1