Hotspot

rps18-rpl20

LSC · 70204-70511 · 308 bp

Back to Parathesis

Pi 0.00325
MarkerSeek score 49.20
Primer available yes
Label rps18-rpl20

Feature Metadata

label_name
rps18-rpl20
feature_type
igs
region
LSC
length_bp
308
variable_sites
1
pi
0.00325
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
49.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps18-rpl20_p1 TGACCTTGAAACAACAACGA GCCGAGTGCTTCTTAATCGA 40.0 50.0 55.8 58.1 519 bp 1.0 61.5
rps18-rpl20_p2 TTGACCTTGAAACAACAACGA GCCGAGTGCTTCTTAATCGA 38.1 50.0 56.5 58.1 520 bp 1.0 59.7
rps18-rpl20_p3 TGACCTTGAAACAACAACGA TTTGTACAAGAGCCGAGTGC 40.0 50.0 55.8 58.5 530 bp 1.0 58.2
rps18-rpl20_p4 TGACCTTGAAACAACAACGAT GCCGAGTGCTTCTTAATCGA 38.1 50.0 56.3 58.1 519 bp 1.0 58.0
rps18-rpl20_p5 TTGACCTTGAAACAACAACGA TTTGTACAAGAGCCGAGTGC 38.1 50.0 56.5 58.5 531 bp 1.0 56.4