Hotspot

trnP-UGG-psaJ

LSC · 68464-68893 · 430 bp

Back to Parathesis

Pi 0.00468

MarkerSeek score 58.60

Primer available yes

Label trnP-UGG-psaJ

Feature Metadata

label_name: trnP-UGG-psaJ
feature_type: igs
region: LSC
length_bp: 430
variable_sites: 2
pi: 0.00468
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 58.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnP-UGG-psaJ_p1	ACCCGTGACATTTTGTACCC	TACTTAGTACCGGTGCCACA	50.0	50.0	58.1	58.1	528-531 bp	1.0	79.3
trnP-UGG-psaJ_p2	CCCGTGACATTTTGTACCCA	TACTTAGTACCGGTGCCACA	50.0	50.0	58.1	58.1	527-530 bp	1.0	79.3
trnP-UGG-psaJ_p3	ACAGGATTTGAACCCGTGAC	TACTTAGTACCGGTGCCACA	50.0	50.0	58.1	58.1	539-542 bp	1.0	79.3
trnP-UGG-psaJ_p4	CAGGATTTGAACCCGTGACA	TACTTAGTACCGGTGCCACA	50.0	50.0	58.1	58.1	538-541 bp	1.0	79.2
trnP-UGG-psaJ_p5	TGACAGGATTTGAACCCGTG	TACTTAGTACCGGTGCCACA	50.0	50.0	58.1	58.1	541-544 bp	1.0	79.2