Hotspot

atpB-rbcL

LSC · 56017-56775 · 759 bp

Back to Parathesis

Pi 0.00132
MarkerSeek score 59.50
Primer available yes
Label atpB-rbcL

Feature Metadata

label_name
atpB-rbcL
feature_type
igs
region
LSC
length_bp
759
variable_sites
1
pi
0.00132
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
59.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
atpB-rbcL_p1 CATCTTACCCGGTGGAAAGG ACACCAGCTTTGAATCCAGT 55.0 45.0 58.0 57.3 929-937 bp 1.0 74.5
atpB-rbcL_p2 ATCTTACCCGGTGGAAAGGA ACACCAGCTTTGAATCCAGT 50.0 45.0 58.0 57.3 928-936 bp 1.0 74.5
atpB-rbcL_p3 TCTTACCCGGTGGAAAGGAT ACACCAGCTTTGAATCCAGT 50.0 45.0 58.0 57.3 927-935 bp 1.0 74.5
atpB-rbcL_p4 TACCCGGTGGAAAGGATACA ACACCAGCTTTGAATCCAGT 50.0 45.0 57.7 57.3 924-932 bp 1.0 72.8
atpB-rbcL_p5 CCCGGTGGAAAGGATACATC ACACCAGCTTTGAATCCAGT 55.0 45.0 57.7 57.3 922-930 bp 1.0 72.7