Hotspot

rpoB-trnC-GCA

LSC · 27517-28650 · 1134 bp

Back to Parathesis

Pi 0.00176

MarkerSeek score 59.80

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 1134
variable_sites: 2
pi: 0.00176
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	CGTCAAGCCCTGATCAATGA	AGCAGAACCGAATAGGGGAT	50.0	50.0	58.0	58.2	1340 bp	1.0	78.4
rpoB-trnC-GCA_p2	CGTCAAGCCCTGATCAATGA	TTATTGATCAGGCGACACCC	50.0	50.0	58.0	57.7	1310 bp	1.0	77.3
rpoB-trnC-GCA_p3	CGTCAAGCCCTGATCAATGA	AAAAGGATTTGCAGTCCCCC	50.0	50.0	58.0	58.4	1274 bp	1.0	77.1
rpoB-trnC-GCA_p4	AGAAAGCTCTTCCGTCAAGC	AGCAGAACCGAATAGGGGAT	50.0	50.0	58.2	58.2	1352 bp	1.0	77.0
rpoB-trnC-GCA_p5	CGTCAAGCCCTGATCAATGA	GCGACACCCAGATTTGAACT	50.0	50.0	58.0	58.5	1299 bp	1.0	76.1