Hotspot

trnS-GCU-trnS-GCA

LSC · 9168-9920 · 753 bp

Back to Parathesis

Pi 0

MarkerSeek score 59.30

Primer available yes

Label trnS-GCU-trnS-GCA

Feature Metadata

label_name: trnS-GCU-trnS-GCA
feature_type: igs
region: LSC
length_bp: 753
variable_sites: 0
pi: 0
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 59.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnS-GCA_p1	GATTCGAACCCTCGGTACAA	AGACCGAAAGACCCCTTAAC	50.0	50.0	57.3	56.9	899-903 bp	1.0	65.9
trnS-GCU-trnS-GCA_p2	GATTCGAACCCTCGGTACAA	GACCGAAAGACCCCTTAACT	50.0	50.0	57.3	56.9	898-902 bp	1.0	65.9
trnS-GCU-trnS-GCA_p3	AACAAAGTCATCGAGGGAGG	AGACCGAAAGACCCCTTAAC	50.0	50.0	57.2	56.9	931-935 bp	1.0	65.0
trnS-GCU-trnS-GCA_p4	AACAAAGTCATCGAGGGAGG	GACCGAAAGACCCCTTAACT	50.0	50.0	57.2	56.9	930-934 bp	1.0	65.0
trnS-GCU-trnS-GCA_p5	GATTCGAACCCTCGGTACAA	AGACCGAAAGACCCCTTAACT	50.0	47.6	57.3	58.4	899-903 bp	1.0	64.0