Hotspot

ycf1

SSC · 122311-127869 · 5559 bp

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Pi 0.0205

MarkerSeek score 51.30

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5559
variable_sites: 113
pi: 0.0205
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 51.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	CCAAAAGTTGCCAAACTGGG	TTTTGCGGGACAGCTCATAA	50.0	45.0	58.0	57.8	1158-1167 bp	1.0	80.7
ycf1_p2	CCAAAAGTTGCCAAACTGGG	TTTTTGCGGGACAGCTCATA	50.0	45.0	58.0	57.8	1159-1168 bp	1.0	80.7
ycf1_p3	TGGTCGTCTCCTTCGGTTAT	TTTTGCGGGACAGCTCATAA	50.0	45.0	58.2	57.8	1024-1033 bp	1.0	80.1
ycf1_p4	CGGTGGATACTGCTTCTTCC	TTTTGCGGGACAGCTCATAA	55.0	45.0	58.1	57.8	935 bp	0.5	58.0
ycf1_p5	CGGTGGATACTGCTTCTTCC	TTTTTGCGGGACAGCTCATA	55.0	45.0	58.1	57.8	936 bp	0.5	58.0