Hotspot

clpP

LSC · 71534-72921 · 1388 bp

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Pi 0.057

MarkerSeek score 73.50

Primer available yes

Label clpP

Feature Metadata

label_name: clpP
feature_type: gene
region: LSC
length_bp: 1388
variable_sites: 79
pi: 0.057
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 73.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP_p1	GATTGGCTGTCTTGCGTTTC	TCCAAAAACCCGGATTCGTT	50.0	45.0	58.0	58.0	2317-2339 bp	1.0	84.2
clpP_p2	TGCACCAACGGAAACCATAA	TCCAAAAACCCGGATTCGTT	45.0	45.0	58.0	58.0	2547-2569 bp	1.0	83.9
clpP_p3	TTGCACCAACGGAAACCATA	TCCAAAAACCCGGATTCGTT	45.0	45.0	58.0	58.0	2548-2570 bp	1.0	83.9
clpP_p4	GGTCCCTTCTATACACCGGA	TCCAAAAACCCGGATTCGTT	55.0	45.0	57.9	58.0	2849-2871 bp	1.0	83.0
clpP_p5	AGGTCCCTTCTATACACCGG	TCCAAAAACCCGGATTCGTT	55.0	45.0	57.9	58.0	2850-2872 bp	1.0	83.0