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rps15-ycf1

SSC · 121913-122310 · 398 bp

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Pi 0.0286

MarkerSeek score 68.40

Primer available yes

Label rps15-ycf1

Feature Metadata

label_name: rps15-ycf1
feature_type: igs
region: SSC
length_bp: 398
variable_sites: 11
pi: 0.0286
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 68.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps15-ycf1_p1	GACCCCCTGTTTTCTTCGTT	ATGATGGCAGTCGTTTCAGT	50.0	45.0	58.0	57.5	509-522 bp	1.0	79.7
rps15-ycf1_p2	GACCCCCTGTTTTCTTCGTT	TGGCAGTCGTTTCAGTATGT	50.0	45.0	58.0	57.2	505-518 bp	1.0	76.9
rps15-ycf1_p3	TTGAAATTCAACGGACCCCC	ATGATGGCAGTCGTTTCAGT	50.0	45.0	58.4	57.5	522-535 bp	1.0	76.8
rps15-ycf1_p4	ACCCCCTGTTTTCTTCGTTT	ATGATGGCAGTCGTTTCAGT	45.0	45.0	57.6	57.5	508-521 bp	1.0	76.3
rps15-ycf1_p5	GACCCCCTGTTTTCTTCGTT	TGATGGCAGTCGTTTCAGTA	50.0	45.0	58.0	56.9	508-521 bp	1.0	74.6