Hotspot

rpl23-trnI-CAU

IRb · 85956-86300 · 345 bp

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Pi 0.0421

MarkerSeek score 65.30

Primer available yes

Label rpl23-trnI-CAU

Feature Metadata

label_name: rpl23-trnI-CAU
feature_type: igs
region: IRb
length_bp: 345
variable_sites: 8
pi: 0.0421
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.55
markerseek_score: 65.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl23-trnI-CAU_p1	TGATTTTTCGCCAATAACCGA	GAATGGTTAAAGCGCCCAAC	38.1	50.0	56.5	58.0	314-469 bp	1.0	65.8
rpl23-trnI-CAU_p2	TGATTTTTCGCCAATAACCGA	CCTGTTAAGCATCCATGGCT	38.1	50.0	56.5	57.9	334-489 bp	1.0	65.3
rpl23-trnI-CAU_p3	CCTAGTTGATCCTGATTCGACA	GAATGGTTAAAGCGCCCAAC	45.5	50.0	57.3	58.0	348-503 bp	1.0	64.2
rpl23-trnI-CAU_p4	TGTCCTAGTTGATCCTGATTCG	GAATGGTTAAAGCGCCCAAC	45.5	50.0	57.3	58.0	351-506 bp	1.0	64.2
rpl23-trnI-CAU_p5	CGACCCAATGCTTTATTTCTGT	GAATGGTTAAAGCGCCCAAC	40.9	50.0	57.3	58.0	370-525 bp	1.0	63.7