Hotspot

rps12-clpP

LSC · 71362-71533 · 172 bp

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Pi 0.0872
MarkerSeek score 73.50
Primer available yes
Label rps12-clpP

Feature Metadata

label_name
rps12-clpP
feature_type
igs
region
LSC
length_bp
172
variable_sites
15
pi
0.0872
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
73.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps12-clpP_p1 GATTGGCTGTCTTGCGTTTC TGGAATTATTGATCTTATAGCAGA 50.0 29.2 58.0 53.4 245-258 bp 1.0 44.8
rps12-clpP_p2 AGGACATCCCCTAAGAGCAG TGGAATTATTGATCTTATAGCAGA 55.0 29.2 58.2 53.4 284-297 bp 1.0 44.1
rps12-clpP_p3 GAGGACATCCCCTAAGAGCA TGGAATTATTGATCTTATAGCAGA 55.0 29.2 58.2 53.4 285-298 bp 1.0 44.1
rps12-clpP_p4 CTCGTACATGTTCCTCGACG TGGAATTATTGATCTTATAGCAGA 55.0 29.2 58.2 53.4 307-320 bp 1.0 43.8
rps12-clpP_p5 CCTCGTACATGTTCCTCGAC TGGAATTATTGATCTTATAGCAGA 55.0 29.2 57.8 53.4 308-321 bp 1.0 43.8