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trnE-UUC-psbD

LSC · 30915-33258 · 2344 bp

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Pi 0.0202

MarkerSeek score 71.20

Primer available yes

Label trnE-UUC-psbD

Feature Metadata

label_name: trnE-UUC-psbD
feature_type: igs
region: LSC
length_bp: 2344
variable_sites: 46
pi: 0.0202
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 71.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnE-UUC-psbD_p1	CGTAGTATCCTACCCCCAGG	GAAACGGTCCCTACGTAACC	60.0	55.0	58.1	58.0	2511-2601 bp	1.0	82.8
trnE-UUC-psbD_p2	GCTGCCTCCTTGAAAGAGAG	GAAACGGTCCCTACGTAACC	55.0	55.0	58.3	58.0	2476-2566 bp	1.0	81.4
trnE-UUC-psbD_p3	TGTCCTGAACCACTAGACGA	GAAACGGTCCCTACGTAACC	50.0	55.0	57.7	58.0	2455-2545 bp	1.0	81.2
trnE-UUC-psbD_p4	CGTAGTATCCTACCCCCAGG	ACAAAAACGAAACGGTCCCT	60.0	45.0	58.1	58.2	2519-2609 bp	1.0	80.8
trnE-UUC-psbD_p5	CGTAGTATCCTACCCCCAGG	GACCGGACCAACCTACAAAA	60.0	50.0	58.1	57.7	2533-2623 bp	1.0	80.7