Hotspot

rps16-trnQ-UUG

LSC · 6210-7976 · 1767 bp

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Pi 0.0445

MarkerSeek score 79.20

Primer available yes

Label rps16-trnQ-UUG

Feature Metadata

label_name: rps16-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 1767
variable_sites: 65
pi: 0.0445
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.83
markerseek_score: 79.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	AAGTCGCACGTTGCTTTCTA	TTTGGTCCCGGTGATCAAAG	45.0	50.0	58.1	58.1	1579-1857 bp	1.0	85.1
rps16-trnQ-UUG_p2	TCCACAGTCGATCATGTCCT	TTTGGTCCCGGTGATCAAAG	50.0	50.0	58.1	58.1	1601-1879 bp	1.0	85.0
rps16-trnQ-UUG_p3	TCATGTCCTTCAAGTCGCAC	TTTGGTCCCGGTGATCAAAG	50.0	50.0	58.2	58.1	1590-1868 bp	1.0	84.6
rps16-trnQ-UUG_p4	AAGTCGCACGTTGCTTTCTA	CCGGTGATCAAAGGTTCGAA	45.0	50.0	58.1	58.2	1572-1850 bp	1.0	84.4
rps16-trnQ-UUG_p5	TCCACAGTCGATCATGTCCT	CCGGTGATCAAAGGTTCGAA	50.0	50.0	58.1	58.2	1594-1872 bp	1.0	84.2