Hotspot

rbcL

LSC · 54765-56243 · 1479 bp

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Pi 0.00316

MarkerSeek score 60.80

Primer available yes

Label rbcL

Feature Metadata

label_name: rbcL
feature_type: gene
region: LSC
length_bp: 1479
variable_sites: 7
pi: 0.00316
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL_p1	ACGCCTAATTCATGTCGAGT	TGGGCCGAGTTTCATTGAAA	45.0	45.0	57.0	58.0	1569 bp	1.0	72.6
rbcL_p2	ACGCCTAATTCATGTCGAGT	TTGGGCCGAGTTTCATTGAA	45.0	45.0	57.0	58.0	1570 bp	1.0	72.6
rbcL_p3	ACGCCTAATTCATGTCGAGT	ATTGGGCCGAGTTTCATTGA	45.0	45.0	57.0	57.8	1571 bp	1.0	70.9
rbcL_p4	ACGCCTAATTCATGTCGAGT	GTTGGATTGGATTCGGCTCA	45.0	50.0	57.0	58.2	1607 bp	1.0	70.7
rbcL_p5	ACGCCTAATTCATGTCGAGT	TGTTGGATTGGATTCGGCTC	45.0	50.0	57.0	58.2	1608 bp	1.0	70.7