Hotspot

rps15-ycf1

SSC · 121686-126397 · 4712 bp

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Pi 0.00156

MarkerSeek score 38.90

Primer available yes

Label rps15-ycf1

Feature Metadata

label_name: rps15-ycf1
feature_type: igs
region: SSC
length_bp: 4712
variable_sites: 11
pi: 0.00156
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 38.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps15-ycf1_p1	TCTGGAGAATTAGGAAAGGGA	AGAATTGAGGCTTTAGAGACTGT	42.9	39.1	55.4	57.2	305 bp	1.0	40.5
rps15-ycf1_p2	CTGGAGAATTAGGAAAGGGAAGA	AGAATTGAGGCTTTAGAGACTGT	43.5	39.1	57.3	57.2	304 bp	1.0	40.5
rps15-ycf1_p3	TCTGGAGAATTAGGAAAGGGAAG	AGAATTGAGGCTTTAGAGACTGT	43.5	39.1	57.3	57.2	305 bp	1.0	40.5
rps15-ycf1_p4	TCTGGAGAATTAGGAAAGGGAA	AGAATTGAGGCTTTAGAGACTGT	40.9	39.1	56.2	57.2	305 bp	1.0	40.5
rps15-ycf1_p5	TGGAGAATTAGGAAAGGGAAGA	AGAATTGAGGCTTTAGAGACTGT	40.9	39.1	56.2	57.2	303 bp	1.0	40.5