Hotspot

rpl16-rps3

LSC · 80712-81947 · 1236 bp

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Pi 0.00216

MarkerSeek score 60.10

Primer available yes

Label rpl16-rps3

Feature Metadata

label_name: rpl16-rps3
feature_type: igs
region: LSC
length_bp: 1236
variable_sites: 4
pi: 0.00216
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16-rps3_p1	CTAGATGTGATCCAAGCGGG	GAGGGTAGGGTTCCACTACA	55.0	55.0	57.8	57.8	1439 bp	1.0	77.0
rpl16-rps3_p2	GGCGACCTGCTTCTATTTGT	GAGGGTAGGGTTCCACTACA	50.0	55.0	58.3	57.8	1459 bp	1.0	76.2
rpl16-rps3_p3	GTTCAAGTGCCTGAAGAGCA	GAGGGTAGGGTTCCACTACA	50.0	55.0	58.4	57.8	1420 bp	1.0	75.1
rpl16-rps3_p4	CTAGATGTGATCCAAGCGGG	AGAGGGTAGGGTTCCACTAC	55.0	55.0	57.8	57.5	1440 bp	1.0	74.6
rpl16-rps3_p5	GGGTTCAAGTGCCTGAAGAG	GAGGGTAGGGTTCCACTACA	55.0	55.0	58.5	57.8	1422 bp	1.0	74.5