Hotspot

psaI-ycf4

LSC · 59046-59435 · 390 bp

Back to Parasenecio

Pi 0.0094

MarkerSeek score 60.20

Primer available yes

Label psaI-ycf4

Feature Metadata

label_name: psaI-ycf4
feature_type: igs
region: LSC
length_bp: 390
variable_sites: 5
pi: 0.0094
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaI-ycf4_p1	GCAATCGCAATGGCTTCTTT	TACTTGTTTTTCGAGCCCCC	45.0	50.0	58.0	58.1	505-507 bp	1.0	80.0
psaI-ycf4_p2	GCAATCGCAATGGCTTCTTT	GGATAACAGCCCAGCAGAAA	45.0	50.0	58.0	57.9	526-528 bp	1.0	79.7
psaI-ycf4_p3	GCAATCGCAATGGCTTCTTT	AAAGGATAACAGCCCAGCAG	45.0	50.0	58.0	57.9	529-531 bp	1.0	79.7
psaI-ycf4_p4	TAGGCCTAGTATTTCCGGCA	TACTTGTTTTTCGAGCCCCC	50.0	50.0	57.9	58.1	522-524 bp	1.0	79.2
psaI-ycf4_p5	TAGGCCTAGTATTTCCGGCA	GGATAACAGCCCAGCAGAAA	50.0	50.0	57.9	57.9	543-545 bp	1.0	79.0