Hotspot

psbK-psbI

LSC · 7928-8378 · 451 bp

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Pi 0.00444

MarkerSeek score 52.80

Primer available yes

Label psbK-psbI

Feature Metadata

label_name: psbK-psbI
feature_type: igs
region: LSC
length_bp: 451
variable_sites: 3
pi: 0.00444
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 52.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbK-psbI_p1	GTTTGGCAAGCTGCTGTAAG	TCTTCACGTCCTGGATTACG	50.0	50.0	57.9	57.1	587-588 bp	1.0	72.6
psbK-psbI_p2	TGGCAAGCTGCTGTAAGTTT	TCTTCACGTCCTGGATTACG	45.0	50.0	58.3	57.1	584-585 bp	1.0	70.6
psbK-psbI_p3	TTGGCAAGCTGCTGTAAGTT	TCTTCACGTCCTGGATTACG	45.0	50.0	58.3	57.1	585-586 bp	1.0	70.6
psbK-psbI_p4	TTTGGCAAGCTGCTGTAAGT	TCTTCACGTCCTGGATTACG	45.0	50.0	58.3	57.1	586-587 bp	1.0	70.6
psbK-psbI_p5	GTTTGGCAAGCTGCTGTAAG	TTCTTCACGTCCTGGATTACG	50.0	47.6	57.9	57.8	588-589 bp	1.0	70.3