Hotspot

rps16

LSC · 5192-6301 · 1110 bp

Back to Parasassafras

Pi 0

MarkerSeek score 40.00

Primer available yes

Label rps16

Feature Metadata

label_name: rps16
feature_type: gene
region: LSC
length_bp: 1110
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 40.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16_p1	GGGTCATGTGAGAGCAACAA	ACCGATTGAGCCAATGACTA	50.0	45.0	58.1	56.6	1290 bp	1.0	68.2
rps16_p2	GGGTCATGTGAGAGCAACAA	TACCGATTGAGCCAATGACT	50.0	45.0	58.1	56.6	1291 bp	1.0	68.2
rps16_p3	TGATACGGGGTCATGTGAGA	ACCGATTGAGCCAATGACTA	50.0	45.0	57.8	56.6	1297 bp	1.0	67.8
rps16_p4	TGATACGGGGTCATGTGAGA	TACCGATTGAGCCAATGACT	50.0	45.0	57.8	56.6	1298 bp	1.0	67.8
rps16_p5	GGGTCATGTGAGAGCAACAA	TCAATTCCATACCGGTCCAA	50.0	45.0	58.1	56.5	1251 bp	1.0	67.3