Hotspot

atpF

LSC · 13062-14342 · 1281 bp

Back to Parasassafras

Pi 0

MarkerSeek score 40.00

Primer available yes

Label atpF

Feature Metadata

label_name: atpF
feature_type: gene
region: LSC
length_bp: 1281
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 40.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF_p1	ACTAATTTCGTCGGCTCGAA	CAAAGGGGGCGAAGTGATAA	45.0	50.0	57.4	57.9	1449 bp	1.0	73.9
atpF_p2	ACTAATTTCGTCGGCTCGAA	CAATCCAAAACAAAGGGGGC	45.0	50.0	57.4	57.8	1459 bp	1.0	73.4
atpF_p3	AATTTCGTCGGCTCGAATGG	CAAAGGGGGCGAAGTGATAA	50.0	50.0	59.0	57.9	1446 bp	1.0	71.1
atpF_p4	AATTTCGTCGGCTCGAATGG	CAATCCAAAACAAAGGGGGC	50.0	50.0	59.0	57.8	1456 bp	1.0	70.6
atpF_p5	ACTAATTTCGTCGGCTCGAAT	CAAAGGGGGCGAAGTGATAA	42.9	50.0	57.8	57.9	1449 bp	1.0	69.7