Hotspot

trnS-GCU-trnG-UCC

LSC · 9517-10332 · 816 bp

Back to Parasassafras

Pi 0

MarkerSeek score 40.00

Primer available yes

Label trnS-GCU-trnG-UCC

Feature Metadata

label_name: trnS-GCU-trnG-UCC
feature_type: igs
region: LSC
length_bp: 816
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 40.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnG-UCC_p1	ATTAGCAATCCGCCGCTTTA	ACGAATCACGCTTTTACCACT	45.0	42.9	58.0	58.2	890 bp	1.0	69.8
trnS-GCU-trnG-UCC_p2	ATTAGCAATCCGCCGCTTTA	ACGAATCACGCTTTTACCAC	45.0	45.0	58.0	56.8	890 bp	1.0	69.7
trnS-GCU-trnG-UCC_p3	ATTAGCAATCCGCCGCTTTA	AACGAATCACGCTTTTACCAC	45.0	42.9	58.0	57.5	891 bp	1.0	67.4
trnS-GCU-trnG-UCC_p4	ATTAGCAATCCGCCGCTTTA	CGAATCACGCTTTTACCACT	45.0	45.0	58.0	56.5	889 bp	1.0	67.4
trnS-GCU-trnG-UCC_p5	GCTTTAGTCCACTCAGCCAT	ACGAATCACGCTTTTACCACT	50.0	42.9	57.6	58.2	876 bp	1.0	66.8