Hotspot

ndhF

SSC · 115907-118135 · 2229 bp

Back to Paraquilegia

Pi 0.00559

MarkerSeek score 54.60

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2229
variable_sites: 12
pi: 0.00559
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 54.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	AAAAGAGAAAAACCTCTGGT	ATATAAGAGCCGGTGAATCG	35.0	45.0	52.5	54.8	2319 bp	0.5	20.0
ndhF_p2	AAAAAGAGAAAAACCTCTGGT	ATATAAGAGCCGGTGAATCG	33.3	45.0	53.4	54.8	2320 bp	0.5	20.0
ndhF_p3	AAAAGAGAAAAACCTCTGGT	CGGTGAATCGGAAACAATTA	35.0	40.0	52.5	54.0	2309 bp	0.5	20.0
ndhF_p4	AAAAGAGAAAAACCTCTGGT	CGGTGAATCGGAAACAATTAAAC	35.0	39.1	52.5	57.0	2309 bp	0.5	20.0
ndhF_p5	AAAAAGAGAAAAACCTCTGGT	CGGTGAATCGGAAACAATTA	33.3	40.0	53.4	54.0	2310 bp	0.5	20.0