Hotspot

ccsA

SSC · 119421-120404 · 984 bp

Back to Paraquilegia

Pi 0.00307

MarkerSeek score 53.20

Primer available yes

Label ccsA

Feature Metadata

label_name: ccsA
feature_type: gene
region: SSC
length_bp: 984
variable_sites: 3
pi: 0.00307
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 53.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA_p1	TCCATTGTTGGGAGACATCC	TGCGTATTGAGTTTGTAAGACCT	50.0	39.1	57.2	58.1	1047-1052 bp	1.0	49.7
ccsA_p2	CCATTGTTGGGAGACATCCT	TGCGTATTGAGTTTGTAAGACCT	50.0	39.1	57.2	58.1	1046-1051 bp	1.0	49.6
ccsA_p3	TCCATTGTTGGGAGACATCC	TGCGTATTGAGTTTGTAAGACC	50.0	40.9	57.2	56.7	1047-1052 bp	1.0	48.4
ccsA_p4	CCATTGTTGGGAGACATCCT	TGCGTATTGAGTTTGTAAGACC	50.0	40.9	57.2	56.7	1046-1051 bp	1.0	48.4
ccsA_p5	TTCCATTGTTGGGAGACATCC	TGCGTATTGAGTTTGTAAGACCT	47.6	39.1	57.9	58.1	1048-1053 bp	1.0	47.4