Hotspot

trnE-UUC-trnT-GGU

LSC · 31712-32381 · 670 bp

Back to Paraquilegia

Pi 0.00604

MarkerSeek score 57.60

Primer available yes

Label trnE-UUC-trnT-GGU

Feature Metadata

label_name: trnE-UUC-trnT-GGU
feature_type: igs
region: LSC
length_bp: 670
variable_sites: 4
pi: 0.00604
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 57.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnE-UUC-trnT-GGU_p1	ACACGATCAACCTCCTTTCG	GAACCGATGACTTACGCCTT	50.0	50.0	57.9	58.0	818-826 bp	1.0	80.5
trnE-UUC-trnT-GGU_p2	CACGATCAACCTCCTTTCGT	GAACCGATGACTTACGCCTT	50.0	50.0	57.9	58.0	817-825 bp	1.0	80.5
trnE-UUC-trnT-GGU_p3	TTTCGTAGTACCCTACCCCC	GAACCGATGACTTACGCCTT	55.0	50.0	57.8	58.0	803-811 bp	1.0	79.9
trnE-UUC-trnT-GGU_p4	ACACGATCAACCTCCTTTCG	TGGAAAAAGCCCCTTATCGG	50.0	50.0	57.9	57.9	842-850 bp	1.0	79.4
trnE-UUC-trnT-GGU_p5	CACGATCAACCTCCTTTCGT	TGGAAAAAGCCCCTTATCGG	50.0	50.0	57.9	57.9	841-849 bp	1.0	79.4