Hotspot

atpH-atpI

LSC · 13265-14227 · 963 bp

Back to Paraquilegia

Pi 0.00313

MarkerSeek score 61.20

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 963
variable_sites: 3
pi: 0.00313
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 61.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	TCCAGGTCCAATAGAAGCCA	ATAGGGGAATCCATGGAGGG	50.0	55.0	58.0	57.9	1068 bp	1.0	79.9
atpH-atpI_p2	ATAACGGAAGCGGCAGAAAT	ATAGGGGAATCCATGGAGGG	45.0	55.0	58.0	57.9	1025 bp	1.0	79.9
atpH-atpI_p3	TACCTTGACCAACTCCAGGT	ATAGGGGAATCCATGGAGGG	50.0	55.0	57.9	57.9	1081 bp	1.0	79.2
atpH-atpI_p4	GATACCTTCTACGGCTTGGC	ATAGGGGAATCCATGGAGGG	55.0	55.0	58.1	57.9	1110 bp	1.0	79.1
atpH-atpI_p5	CGATACCTTCTACGGCTTGG	ATAGGGGAATCCATGGAGGG	55.0	55.0	57.9	57.9	1111 bp	1.0	79.0