Hotspot

trnK-UUU-rps16

LSC · 4180-4878 · 699 bp

Back to Paraquilegia

Pi 0.0219

MarkerSeek score 73.20

Primer available yes

Label trnK-UUU-rps16

Feature Metadata

label_name: trnK-UUU-rps16
feature_type: igs
region: LSC
length_bp: 699
variable_sites: 15
pi: 0.0219
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 73.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rps16_p1	TCTACCAACGGTATGGACGA	CCTTGAAAAAGGCGCTCAAC	50.0	50.0	57.9	58.2	892-910 bp	1.0	79.7
trnK-UUU-rps16_p2	TCTACCAACGGTATGGACGA	CTTGAAAAAGGCGCTCAACC	50.0	50.0	57.9	58.2	891-909 bp	1.0	79.7
trnK-UUU-rps16_p3	AAGCCGAGTACTCTACCGTT	CCTTGAAAAAGGCGCTCAAC	50.0	50.0	58.2	58.2	826-844 bp	1.0	79.6
trnK-UUU-rps16_p4	AAGCCGAGTACTCTACCGTT	CTTGAAAAAGGCGCTCAACC	50.0	50.0	58.2	58.2	825-843 bp	1.0	79.6
trnK-UUU-rps16_p5	TCTACCAACGGTATGGACGA	TGAAAAAGGCGCTCAACCTA	50.0	45.0	57.9	57.7	889-907 bp	1.0	79.5