Hotspot

rrn16

IRb · 99805-101294 · 1490 bp

Back to Paraprenanthes

Pi 0

MarkerSeek score 38.90

Primer available yes

Label rrn16

Feature Metadata

label_name: rrn16
feature_type: rRNA
region: IRb
length_bp: 1490
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 38.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rrn16_p1	CGAATCCGCTTTGTCTACGA	AGCTCCCTTCTTTTTGGGTG	50.0	50.0	58.1	58.0	1610 bp	1.0	79.2
rrn16_p2	CGAATCCGCTTTGTCTACGA	AACTCAGACGTAGCTCCCTT	50.0	50.0	58.1	58.1	1621 bp	1.0	78.6
rrn16_p3	CGAATCCGCTTTGTCTACGA	TGTGAAGCAGTGTCAAACCA	50.0	45.0	58.1	57.9	1592 bp	1.0	78.3
rrn16_p4	GAACTCCGGGCGAATATGAA	AGCTCCCTTCTTTTTGGGTG	50.0	50.0	57.8	58.0	1671 bp	1.0	78.1
rrn16_p5	AACTCCGGGCGAATATGAAG	AGCTCCCTTCTTTTTGGGTG	50.0	50.0	57.8	58.0	1670 bp	1.0	78.1