Hotspot

trnF-ndhJ

LSC · 47837-48467 · 631 bp

Back to Paraprenanthes

Pi 0.00238

MarkerSeek score 60.90

Primer available yes

Label trnF-ndhJ

Feature Metadata

label_name: trnF-ndhJ
feature_type: igs
region: LSC
length_bp: 631
variable_sites: 3
pi: 0.00238
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-ndhJ_p1	CCTCGTGTCACCAGTTCAAA	TGGATAGGATGGCCTTTACG	50.0	50.0	58.1	56.8	731-734 bp	1.0	70.5
trnF-ndhJ_p2	CCTCGTGTCACCAGTTCAAA	GGATAGGATGGCCTTTACGT	50.0	50.0	58.1	56.8	730-733 bp	1.0	70.5
trnF-ndhJ_p3	CCTCGTGTCACCAGTTCAAA	TGAAAGTTGGATAGGATGGCC	50.0	47.6	58.1	57.7	738-741 bp	1.0	69.9
trnF-ndhJ_p4	CCTCGTGTCACCAGTTCAAA	TTGGATAGGATGGCCTTTACG	50.0	47.6	58.1	57.5	732-735 bp	1.0	68.4
trnF-ndhJ_p5	CCTCGTGTCACCAGTTCAAA	TGGATAGGATGGCCTTTACGT	50.0	47.6	58.1	58.6	731-734 bp	1.0	67.5