Hotspot

trnT-psbD

LSC · 31110-32326 · 1217 bp

Back to Paraprenanthes

Pi 0.00137

MarkerSeek score 59.70

Primer available yes

Label trnT-psbD

Feature Metadata

label_name: trnT-psbD
feature_type: igs
region: LSC
length_bp: 1217
variable_sites: 3
pi: 0.00137
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-psbD_p1	CGTAAGCCCTCGGTTCAAAT	GGACCAGCCTACAAAAACGA	50.0	50.0	58.3	58.1	1352 bp	1.0	77.1
trnT-psbD_p2	TCCATGAACCAAGCCCTTTT	GGACCAGCCTACAAAAACGA	45.0	50.0	57.6	58.1	1408 bp	1.0	76.1
trnT-psbD_p3	TTCCATGAACCAAGCCCTTT	GGACCAGCCTACAAAAACGA	45.0	50.0	57.6	58.1	1409 bp	1.0	76.1
trnT-psbD_p4	CGTAAGCCCTCGGTTCAAAT	ACAAAAACGAAACGGTCCCT	50.0	45.0	58.3	58.2	1342 bp	1.0	76.1
trnT-psbD_p5	TAAGCCCTCGGTTCAAATCC	GGACCAGCCTACAAAAACGA	50.0	50.0	57.6	58.1	1350 bp	1.0	76.0