Hotspot

trnC-GCA-petN

LSC · 9449-10271 · 823 bp

Back to Paraprenanthes

Pi 0.00972

MarkerSeek score 64.70

Primer available yes

Label trnC-GCA-petN

Feature Metadata

label_name: trnC-GCA-petN
feature_type: igs
region: LSC
length_bp: 823
variable_sites: 14
pi: 0.00972
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-petN_p1	TTTTGTATCGTTTTGGCGGC	AGCCCAAGCGAGACTTACTA	45.0	50.0	57.9	58.2	933-946 bp	1.0	80.1
trnC-GCA-petN_p2	TTTCCCCAGTTCAAATCCGG	AGCCCAAGCGAGACTTACTA	50.0	50.0	58.1	58.2	877-890 bp	1.0	79.9
trnC-GCA-petN_p3	GGGGGACTGCAAATCCTTTT	AGCCCAAGCGAGACTTACTA	50.0	50.0	58.4	58.2	895-908 bp	1.0	77.7
trnC-GCA-petN_p4	TTTTGTATCGTTTTGGCGGC	GCCCAAGCGAGACTTACTAT	45.0	50.0	57.9	57.1	932-945 bp	1.0	74.1
trnC-GCA-petN_p5	TTTTGTATCGTTTTGGCGGC	ATTAAAGCAGCCCAAGCGAG	45.0	50.0	57.9	58.9	941-954 bp	1.0	74.1