Hotspot

ycf3

LSC · 42463-44420 · 1958 bp

Back to Paraphlomis

Pi 0.00257

MarkerSeek score 55.90

Primer available yes

Label ycf3

Feature Metadata

label_name: ycf3
feature_type: gene
region: LSC
length_bp: 1958
variable_sites: 39
pi: 0.00257
unique_haplotype_count: 25
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 55.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf3_p1	TTGACCAATGAGGCCAAAAA	TCCAAAGAAAGGGAATGAGAAGA	40.0	39.1	56.0	57.4	2115-2125 bp	1.0	41.9
ycf3_p2	TTTGACCAATGAGGCCAAAA	TCCAAAGAAAGGGAATGAGAAGA	40.0	39.1	56.0	57.4	2116-2126 bp	1.0	41.8
ycf3_p3	TTTGACCAATGAGGCCAAAAA	TCCAAAGAAAGGGAATGAGAAGA	38.1	39.1	56.8	57.4	2116-2126 bp	1.0	41.8
ycf3_p4	TTGACCAATGAGGCCAAAAA	TCTTTGTGGGATCATTTTTCCA	40.0	36.4	56.0	56.1	2133-2143 bp	1.0	41.8
ycf3_p5	TTGACCAATGAGGCCAAAAA	TGTGGGATCATTTTTCCAAAGA	40.0	36.4	56.0	56.1	2129-2139 bp	1.0	41.8