Hotspot

rbcL

LSC · 54946-56400 · 1455 bp

Back to Paraphlomis

Pi 0.00483

MarkerSeek score 53.70

Primer available yes

Label rbcL

Feature Metadata

label_name: rbcL
feature_type: gene
region: LSC
length_bp: 1455
variable_sites: 34
pi: 0.00483
unique_haplotype_count: 19
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 53.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL_p1	AACGCCCAATTCATTCGTGT	TGGGCCGAGTTTAATTGCAA	45.0	45.0	58.8	58.4	1558-1575 bp	1.0	73.5
rbcL_p2	AACGCCCAATTCATTCGTGT	TTGGGCCGAGTTTAATTGCA	45.0	45.0	58.8	58.4	1559-1576 bp	1.0	73.5
rbcL_p3	GCCCAATTCATTCGTGTCGA	TGGGCCGAGTTTAATTGCAA	50.0	45.0	58.9	58.4	1555-1572 bp	1.0	72.2
rbcL_p4	GCCCAATTCATTCGTGTCGA	TTGGGCCGAGTTTAATTGCA	50.0	45.0	58.9	58.4	1556-1573 bp	1.0	72.2
rbcL_p5	TCATGAGTTGTAGGGAGGGA	TGGGCCGAGTTTAATTGCAA	50.0	45.0	57.1	58.4	1505-1522 bp	1.0	72.1