Hotspot

ndhF

SSC · 108935-111157 · 2223 bp

Back to Paraphlomis

Pi 0.0032

MarkerSeek score 40.10

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2223
variable_sites: 51
pi: 0.0032
unique_haplotype_count: 24
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 40.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	ACAGGAACAAGAGGGATCCA	AAAGAGGTAAGGTCGGGTGA	50.0	50.0	58.0	58.0	2513-2521 bp	0.967742	80.3
ndhF_p2	ACAGGAACAAGAGGGATCCA	AAGAGGTAAGGTCGGGTGAA	50.0	50.0	58.0	58.0	2512-2520 bp	0.967742	80.3
ndhF_p3	AAGAGGGATCCACCGAAGAA	AAGAGGTAAGGTCGGGTGAA	50.0	50.0	58.0	58.0	2504-2512 bp	0.967742	80.2
ndhF_p4	ACAGGAACAAGAGGGATCCA	AATCCGGGAAAATATGCCCC	50.0	50.0	58.0	58.0	2731-2738 bp	0.935484	79.3
ndhF_p5	AAGAGGGATCCACCGAAGAA	AATCCGGGAAAATATGCCCC	50.0	50.0	58.0	58.0	2723-2730 bp	0.935484	79.3