Hotspot

matK

LSC · 1906-3441 · 1536 bp

Back to Paraphlomis

Pi 0.00388

MarkerSeek score 56.20

Primer available yes

Label matK

Feature Metadata

label_name: matK
feature_type: gene
region: LSC
length_bp: 1536
variable_sites: 45
pi: 0.00388
unique_haplotype_count: 23
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
matK_p1	GGATCTTTTTATCTCTCGATCCA	AGTTTATCTGTCCCCGAGGT	39.1	50.0	55.6	57.8	1709-1717 bp	1.0	42.5
matK_p2	TGGATCTTTTTATCTCTCGATCC	AGTTTATCTGTCCCCGAGGT	39.1	50.0	55.6	57.8	1710-1718 bp	1.0	42.5
matK_p3	TTGGATCTTTTTATCTCTCGATCC	AGTTTATCTGTCCCCGAGGT	37.5	50.0	56.3	57.8	1711-1719 bp	1.0	42.5
matK_p4	ACATCAACATTTCAGAATAGTGGA	AGTTTATCTGTCCCCGAGGT	33.3	50.0	56.4	57.8	1743-1751 bp	1.0	42.4
matK_p5	ACATTTCAGAATAGTGGAAATCCA	AGTTTATCTGTCCCCGAGGT	33.3	50.0	56.3	57.8	1737-1745 bp	1.0	42.4