Hotspot

ccsA

SSC · 112726-113697 · 972 bp

Back to Paraphlomis

Pi 0.00463

MarkerSeek score 36.30

Primer available yes

Label ccsA

Feature Metadata

label_name: ccsA
feature_type: gene
region: SSC
length_bp: 972
variable_sites: 22
pi: 0.00463
unique_haplotype_count: 15
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 36.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA_p1	GTGAAATCGGTAGACACGCT	TCAGTAGCTTTTCGATGGCC	50.0	50.0	58.0	58.0	1673-1693 bp	1.0	82.5
ccsA_p2	TGAAATCGGTAGACACGCTG	TCAGTAGCTTTTCGATGGCC	50.0	50.0	58.0	58.0	1672-1692 bp	1.0	82.4
ccsA_p3	GTGAAATCGGTAGACACGCT	CAGTAGCTTTTCGATGGCCT	50.0	50.0	58.0	58.0	1672-1692 bp	1.0	82.4
ccsA_p4	TGAAATCGGTAGACACGCTG	CAGTAGCTTTTCGATGGCCT	50.0	50.0	58.0	58.0	1671-1691 bp	1.0	82.4
ccsA_p5	GTGAAATCGGTAGACACGCT	TGACGAAATGGGCGGAATAG	50.0	50.0	58.0	58.1	1723-1743 bp	1.0	82.2