Hotspot

atpF

LSC · 11920-13141 · 1222 bp

Back to Paraphlomis

Pi 0.00383

MarkerSeek score 54.50

Primer available yes

Label atpF

Feature Metadata

label_name: atpF
feature_type: gene
region: LSC
length_bp: 1222
variable_sites: 33
pi: 0.00383
unique_haplotype_count: 21
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF_p1	ATTTCGTCGGCTTGAATGGT	AAGAGGGGCAAAGTGATAGA	45.0	45.0	58.2	55.8	1393-1405 bp	0.967742	62.9
atpF_p2	ATTTCGTCGGCTTGAATGGT	AGAGGGGCAAAGTGATAGAA	45.0	45.0	58.2	55.8	1392-1404 bp	0.967742	62.9
atpF_p3	GTCGGCTTGAATGGTTACCA	AAGAGGGGCAAAGTGATAGA	50.0	45.0	58.2	55.8	1388-1400 bp	0.967742	62.9
atpF_p4	GTCGGCTTGAATGGTTACCA	AGAGGGGCAAAGTGATAGAA	50.0	45.0	58.2	55.8	1387-1399 bp	0.967742	62.9
atpF_p5	TCGTCGGCTTGAATGGTTAC	AGAGGGGCAAAGTGATAGAA	50.0	45.0	58.3	55.8	1389-1401 bp	0.967742	62.1