Hotspot

psaC-ndhE

SSC · 115747-116008 · 262 bp

Back to Paraphlomis

Pi 0.00744

MarkerSeek score 43.00

Primer available yes

Label psaC-ndhE

Feature Metadata

label_name: psaC-ndhE
feature_type: igs
region: SSC
length_bp: 262
variable_sites: 11
pi: 0.00744
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 43.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaC-ndhE_p1	TACATCCGTCCCAGGGTATC	TGAAGCAGCTATTGGATCGG	55.0	50.0	58.0	57.8	456 bp	1.0	80.1
psaC-ndhE_p2	TCCGTCCCAGGGTATCATTT	TGAAGCAGCTATTGGATCGG	50.0	50.0	58.1	57.8	452 bp	1.0	79.3
psaC-ndhE_p3	ATCCGTCCCAGGGTATCATT	TGAAGCAGCTATTGGATCGG	50.0	50.0	57.9	57.8	453 bp	1.0	79.2
psaC-ndhE_p4	TACATCCGTCCCAGGGTATC	GCAGCTATTGGATCGGCTAT	55.0	50.0	58.0	57.6	452 bp	1.0	78.9
psaC-ndhE_p5	CTAATACATCCGTGGGGCAC	TGAAGCAGCTATTGGATCGG	55.0	50.0	58.1	57.8	432 bp	0.967742	77.8