Hotspot

ccsA-ndhD

SSC · 113698-113880 · 183 bp

Back to Paraphlomis

Pi 0.0147

MarkerSeek score 57.90

Primer available yes

Label ccsA-ndhD

Feature Metadata

label_name: ccsA-ndhD
feature_type: igs
region: SSC
length_bp: 183
variable_sites: 11
pi: 0.0147
unique_haplotype_count: 14
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 57.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA-ndhD_p1	AGCAATTGTAGCGTCTATTGGA	CGGTATTGGTATGTACCCCG	40.9	55.0	57.9	57.6	372-380 bp	1.0	65.1
ccsA-ndhD_p2	CAGCAATTGTAGCGTCTATTGG	CGGTATTGGTATGTACCCCG	45.5	55.0	57.8	57.6	373-381 bp	1.0	64.5
ccsA-ndhD_p3	AGCAATTGTAGCGTCTATTGGA	ACCCCGATTTCGTTCTTTCA	40.9	45.0	57.9	57.4	358-366 bp	1.0	64.4
ccsA-ndhD_p4	CAGCAATTGTAGCGTCTATTGG	ACCCCGATTTCGTTCTTTCA	45.5	45.0	57.8	57.4	359-367 bp	1.0	63.9
ccsA-ndhD_p5	ACAAATTCAGCAATTGTAGCGT	CGGTATTGGTATGTACCCCG	36.4	55.0	57.5	57.6	380-388 bp	0.903226	57.9