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psbE-petL

LSC · 64610-65537 · 928 bp

Back to Paraphlomis

Pi 0.00385

MarkerSeek score 61.40

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 928
variable_sites: 24
pi: 0.00385
unique_haplotype_count: 22
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	TGACAAATAACCAACCCGCA	GTCGTATCTTGCTCAGACCA	45.0	50.0	58.0	57.1	1087-1135 bp	1.0	78.1
psbE-petL_p2	CAACCCGCAATGAATAGGGA	GTCGTATCTTGCTCAGACCA	50.0	50.0	57.9	57.1	1076-1124 bp	1.0	77.8
psbE-petL_p3	AGAACGTTCTCCTGTGCTTC	GTCGTATCTTGCTCAGACCA	50.0	50.0	57.8	57.1	999-1047 bp	1.0	77.1
psbE-petL_p4	GTTCTCCTGTGCTTCCAGAC	GTCGTATCTTGCTCAGACCA	55.0	50.0	58.2	57.1	994-1042 bp	1.0	76.8
psbE-petL_p5	ACCAACCCGCAATGAATAGG	GTCGTATCTTGCTCAGACCA	50.0	50.0	58.2	57.1	1078-1126 bp	1.0	76.4