Hotspot

ndhC-trnV-UAC

LSC · 50155-51119 · 965 bp

Back to Paraphlomis

Pi 0.00525
MarkerSeek score 57.20
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
965
variable_sites
34
pi
0.00525
unique_haplotype_count
21
primer_available
yes
alignment_reliability
1.00
markerseek_score
57.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 GGGGCTAAAATCCCGGAAAT AGAAGGTCTACGGTTCGAGT 50.0 50.0 57.9 57.8 1090-1097 bp 1.0 80.8
ndhC-trnV-UAC_p2 TTCTCCGGCCCTTTCCTAAT AGAAGGTCTACGGTTCGAGT 50.0 50.0 58.4 57.8 1111-1118 bp 1.0 78.2
ndhC-trnV-UAC_p3 GGGGCTAAAATCCCGGAAAT GAAGGTCTACGGTTCGAGTC 50.0 55.0 57.9 57.5 1089-1096 bp 1.0 78.1
ndhC-trnV-UAC_p4 GGGGCTAAAATCCCGGAAAT GTTCGAGTCCGTATAGCCCT 50.0 55.0 57.9 58.7 1078-1085 bp 1.0 77.0
ndhC-trnV-UAC_p5 CCCTTTCCTAATCGGGGCTA AGAAGGTCTACGGTTCGAGT 55.0 50.0 58.6 57.8 1103-1110 bp 1.0 76.9